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KMID : 0644320020080010103
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Journal of Korean Oriental Oncology
2002 Volume.8 No. 1 p.103 ~ p.125
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Experimental Studies on the Anti-tumor and the Immunomodulatory Effects of Jiaweicitaowan(Ê¥Ú±í±Óþü¯)
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Jeon Young-Soo
Shim Bum-Sang
Choi Seung-Hun
Ahn Kyoo-Suk
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Abstract
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This experimental study was carried out to evaluate the anti-tumor and the immunomodulatory effects of Jiaweicitaowan(Ê¥Ú±í±Óþü¯) against cancer. The in vitro anti-tumor effects were evaluated by MTT assay. The cytotoxicity, extension of survival days, the effect of inhibition solid tumor which was induced sarcoma 180, and the changes of body weight were evaluated for in vivo effects of anti-tumor. To evaluate the immunomodulatory effects of Jiawei- citaowan(Ê¥Ú±í±Óþü¯), delayed type hypersensitivity, hemagglutinin, hemolysin titers for humoral immune response, rosette forming cells for cell-mediated immune response, natural killer cell activity, proliferation of lymphocyte, productivty of Interleukin-2, and carbon clearance were measured with methotrexate treated mice. The results were as follows; 1. In the case of existence ability of tumor cell, IC50 had an anti-tumor ativity resulted 2.52mg/ml to SNU-C4. 0.41mg/ml to SNU-396, resulted to 0.09mg/mlSNU-1. 2. The groups of Jiaweicitaowan(Ê¥Ú±í±Óþü¯) 10mg/ml, 20mg/kg had no body weight loss. reduction in intake of water and feed, so these had no toxicity. 3. In the case of the effect of extention of existence. the group of 20mg/kg Jiaweicitaowan(Ê¥Ú±í±Óþü¯) extract treated group was showed 250% in ILS. 4. The effect of inhibition solid tumor was significantly decreased in both 10mg/kg, 20mg/kg of Jiaweicitaowan(Ê¥Ú±í±Óþü¯) extract treated groups as compared with control group S. The groups of 10mg/kg, 20mg/kg Jiaweicitaowan(Ê¥Ú±í±Óþü¯) had significant effect of body weight change compared to control group. 6. Delayed type hypersensitivity was not significant in both Jiaweicitaowan(Ê¥Ú±í±Óþü¯) extract treated groups as compared with control group. 7. Hemagglutinin and Hemolysin titers were significantly increased by dose-dependent. so these results showed that the humoral immume respose was activated. 8. For the effect of rosette formimg cells was not significant in hoth Jiaweicitaowan(Ê¥Ú±í±Óþü¯) extract treated groups as compared with control group. 9. Natural killer cell activity was significantly increased in both Jiaweicitaowan(Ê¥Ú±í±Óþü¯) extract treated groups as compared with control group in the ratio of 100: 1, 50: 1 of effector and target cells, but in the ratio of 10:1, the Jiaweicitaowan(Ê¥Ú±í±Óþü¯) extract treated groups were not significant. 10. The proliferation of lymphocyte and productivty of Interleukin-2 were significantly increased by dose-dependent in both 10mg/ kg, 20mg/ kg of Jiaweicitaowan(Ê¥Ú±í±Óþü¯) extract treated groups as compared with control group. 11. In the phagocytic effect, the 20mg/kg of Jiaweicitaowan(Ê¥Ú±í±Óþü¯) extract treated group showed the increasing effect with significance as compared with control group. According to the results, we can suggest that Jiaweicitaowan(Ê¥Ú±í±Óþü¯) has the antitumor and the immunomodulatory effects.
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KEYWORD
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